Exact(3)
Though definitive statements cannot be made, mutation accumulation seems like a plausible cause of the observed acquisition of citrate dependence.
In one case, gaps in the genome and local misassembly would have made mutation identification extremely difficult without exon capture/BSA.
Over the small number of cases were comparisons can be made, mutation rate estimates do not differ between species but do vary significantly depending on the laboratory/operator.
Similar(55)
You'd made mutations look so good".
As a first step towards judging the importance of the exact dEndoA-BAR curvature in vivo, we made mutations designed to induce curvature changes.
We also made mutations at the Q2′ and T6′ positions.
We also made mutations in sptC, homologous to the yeast SAGA component gene SPT3, which showed a similar phenotype.
We therefore made mutations that individually disrupted both of these functions: K125R to prevent carbamylation, and R104A to disrupt formation of the salt bridge.
To test this idea, we made mutations in several putative DDK and CDK sites found in the vicinity of the RVxF and SILK motifs in budding yeast Rif1.
This, in turn, allows for the molecular selection of mutated samples and makes mutation detection in premalignant samples feasible.
Make mutation on selected solutions for mutation to get population (P_{1}(T)).
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