As expected, most of the 96 spots gave hybridization signal with the probe made from the secondary product of the reverse subtracted SSH cDNA (Fig. 1d), but only a few hybridization signals were observed with the probe made from the secondary PCR product of the forward subtracted SSH cDNA (Fig. 1c).
Most of the 150 spotted plasmids from the randomly picked transformed colonies generated for the forward subtracted SSH cDNA showed hybridization signal with the probe made from the secondary PCR product of the forward subtracted SSH cDNA (Fig. 1a).
Upon hybridization of the duplicate blot with the probe made from the secondary PCR product of the reverse subtracted SSH cDNA, only a few hybridization signals were observed (Fig. 1b).
For the screening of the reverse SSH cDNA library, 96 plasmid DNA samples, isolated from the randomly picked transformed bacterial colonies obtained for the reverse subtracted SSH cDNA, were blotted and hybridized with the probe made from the secondary PCR product of either the reverse or the forward subtracted SSH cDNA.
The subtracted cDNA library was made from 100 μL of secondary PCR reaction from gastrula-subtracted cDNA sequences.
Château Haut-Brion and La Mission both have secondary wines made from younger vines, and wines not suited for the two lead properties: Château Bahans de Haut-Brion and La Chapelle du Haut-Brion.
The Air Line Pilots Association has advocated for secondary barriers made from mesh or steel cables to be installed on cockpits doors to make it harder to break into, but airlines have said that they aren't necessary.
Sugar, starch, cotton, linen, hemp, some types of rope, wood and particle boards, papyrus and paper, vegetable oils, wax, and natural rubber are examples of commercially important materials made from plant tissues or their secondary products.
A secondary selection was made from antibodies that scored positive on one or both immunoassays only once.
