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Three DGE libraries were made from replicate cultures for each life cycle stage and sequenced using Solexa (Illumina) technology.
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Plant height was measured in the field as the shortest distance between the uppermost leaves and the soil surface; measurements were made from 25 replicates in each zone (Cornelissen et al. 2003).
An average was made from three technical replicates for each donor.
It will set in 5 to 10 min. The negative impression made from this material replicates the smooth surface of the prosthesis in detail.
Calculations were made from 4 biological replicates VHSV-infected and non-infected each and by 2 independent researchers using Microsoft Excel, Origin pro SR4, BRB-Array Tools and the TIGR Multiple array viewer program (MeV) (see above) and their results confronted until all discrepancies were solved.
In total, 30 expression arrays were run as follows: for each of the 3 Bacillus species, 5 arrays were run for each condition (ambient air versus CO2 for 10 arrays/species), representing 4 biological replicates and one additional replicate representing cDNA made from a mix of RNA from each of 4 biological replicates.
As these 4 libraries were technical replicates made from the same total RNA preparation all other IA levels were similar across all libraries.
Calculations were made from measurements of 3 replicates of each sample.
Replicates of the libraries were made from the overnight cultures and both replicate and master plates were stored in 40% glycerol at -80°C.
Sixty resin (RenCast 52/53 Isocyanatethe FC/FC 52 Polyol) replicates were made from the master model.
Technical replicates were made from the losartan-treated skin resulting in four samples.
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