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Scatter plots were made from normalized signals.
The individual clones from the enriched in vivo cDNA libraries that only hybridized to the probe made from normalized in vivo cDNAs and the individual clones from the enriched in vitro cDNA libraries that only hybridized to the probe made from normalized in vitro cDNAs were chosen for sequence analyses.
The individual clones that only hybridized to the probe made from normalized in vivo cDNAs were chosen for sequence analysis.
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Measurements of polar angle and azimuthal angle were made from the normalized sets and are presented in C – E. (C ) Violin plots of the polar angles for MTs in K-fibers from control and TACC3 overexpressing cells.
Recently we completed a medium scale EST sequencing project from normalized cDNA libraries made from Solanum chacoense ovules in order to identify signal transduction components involved in fertilization and early seed development [ 47].
We generated 33,229 high-quality ESTs from three non-normalized libraries made from the developing eye-stalk tissue at different developmental stages.
As summarized in Table 1, a total of 10,368 clones were sequenced from a normalized Ich library made from pooled cells from all three life cycle stages: theront, tomont, and trophont.
For the determination of the cDNA sequence of csf1ra from a normalized library ("pinky") made from different A. burtoni mRNA pools [ 47], we additionally used the primers listed in Table 3.
We sequenced 10,368 EST clones using a normalized cDNA library made from pooled samples of the trophont, tomont, and theront life-cycle stages, and generated 9,769 sequences (94.2% success rate).
We present here a parallel sequencing effort to produce an extensive EST (expressed sequence tags) dataset derived from a normalized ferret cDNA library made from mRNA from ferret blood, liver, lung, spleen and brain.
Three non-normalized cDNA libraries were made from the eye-antennal imaginal discs and optic lobes of T. dalmanni at three developmental stages: third instar wandering larva (L), early pupae (P1) and mid pupae (P2).
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