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The grape it is made from, known as pinot gris (gray pinot) in France and Ruländer in Germany, grows all over northeastern Italy, with the best coming from Friuli-Venezia or the Alto-Adige.
A dilution series made from known concentrations of plasmid containing the PCR inserts was used to calculate absolute copy numbers for each of the genes examined.
E4 gene copy number was determined using specific E4 primers and TaqMan probes for real-time polymerase chain reaction (PCR) as previously described [28] with TaqMan fast reagents (Applied Biosystems, Framingham, MA) and measured against a standard curve made from known amounts of adenovirus genome.
The standard curve was made from known amounts of the corresponding product with the same primer sets, and was run on each PCR plate.
The plasmid DNA standards were made from known concentrations of plasmid DNA that contained the respective amplicon for each set of primers, according to Bartosch et al. [ 14].
Quotients were scored according to observations made from known samples: wild type if the quotient was between 0.8 and 1.2, hemizygous deletion if the quotient was between 0.4 and 0.7, homozygous deletion if the quotient was between 0.0 and 0.2.
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The Minotaur became a fixture in a series of etchings made from 1930-1937 known as the Vollard Suite, and in the same period he illustrated an edition of Ovid's Metamorphoses.
The chromosome preparations of at least one sample of each species were hybridized with probes made from the known diploid A, B and C species genomes (Table 1).
Biodegradable, organic, and health conscious cosmetics, body and bath products are a burgeoning industry now, including soaps, shampoos, bath oils, bath salts, perfumes, etc., that are made from ingredients known to be safe to both humans and the environment.
We're salivating at the idea of hockey puck tomato slices and mustard made from who knows what. .
Pastes have been made from particles of known size distribution and liquids of known rheology.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com