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There are at least two conclusions that can be made from genomic studies of pancreatic cancer progression.
GS FLX Titanium shotgun libraries were made from genomic DNA according to the manufacturer's specifications at 454 Life Sciences (454 Life Sciences, Branford, CT).
Bisulfite sequencing (WGBS) libraries of the parents and the hybrid were made from genomic DNA isolated from the same leaf tissues as used for RNA-seq libraries.
In our hands, panning libraries made from genomic DNA in a gene III-based vector never gave more than 10-20% positive clones, while gene VIII-based display resulted in almost 100% positive clones (4, 5).
A second PCR product (BCP569-571; Table 2) spanning MATa and adding terminal StuI sites was then made from genomic DNA and cloned into pCR TOPO2.1 (Invitrogen) to produce pBC58, which is available upon request.
Illumina libraries were made from genomic DNA isolated from 10 males from either isogenized Canton-S and w stocks (parental lines) or from the resultant progeny of crosses to C(1 DX females (progeny lines).
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DNA standards were made from total genomic DNA from Y. pestis and were diluted in nuclease-free water to yield a range of 106 to 102 genome copies per 2 µl.
The in-depth analysis of the many tantalizing observations made from comprehensive genomic characterization of breast cancers has barely begun.
Chromosome preparations of the tetraploid species S. plicata were hybridized with probes made from the genomic DNA of S. viridis-Q24, S. adhaerans, S. grisebachii and S. glauca respectively.
The in-depth analysis of the many tantalizing observations made from comprehensive genomic characterization of breast cancers has barely begun and may ultimately represent the most important future contribution of molecular profiling to cancer research.
A tbrd-1-eGFP transgene made from the genomic region (Fig. 1B; Materials and Methods) rescued the sterility of homozygous tbrd-1 males demonstrating that the male infertility was due to the compromised activity of tBRD-1.
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