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Parallel clear uniaxial compression specimens (20 × 20 × 20 mm) were made from control and thermally treated wood, conditioned in a series from an oven dry to saturated atmosphere (20 °C) and compression loaded parallel and transverse to the grain.
The total CLA content in cheese was significantly higher when the CI4b culture was used compared with CLA nonproducing culture cheeses made from control milk and treatment milk after 1 mo [1.09 and 2.14 (±0.18) g of total CLA/100 g of FA] and 6 mo [0.99 and 2.05 (±0.18) g of total CLA/100 g of FA] of ripening, respectively.
The CI4b cheeses made from control milk and treatment milk contained 1.09 and 2.41 (±0.18) g of total CLA/100 g of FA after 1 mo of ripening, which increased to 1.44 and 2.61 (±0.18) g of total CLA/100 g of FA after 6 mo of ripening, respectively.
The results indicated that the combination of a CLA-producing starter culture and milk from cattle fed fish oil-supplemented diets (0.99 g of CLA/100 g of FA) could enhance levels of total CLA in Cheddar cheese by up to 2.6 times compared with cheese made from control milk with CLA nonproducing starter culture (2.61 g of CLA/100 g of FA) after 6 mo.
Transverse sections of the hippocampus (400 µM thickness) were made from control and pilocarpine-treated mice that survived status epilepticus using a vibratome, and the dentate gyrus was micro-dissected to include the molecular layer.
Aliquots of 100 µl of the series made from control and treated organisms were distributed in an opaque black 96-well plate (Corning, NY) and quantified for fluorescent intensity using a FluoroStar Optima plate reader (BMG Labtechnologies, Durham, NC) with an excitation filter of 590 nm and emission of 617 nm.
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Somatic whole-cell patch-clamp recordings were made from healthy (control), nonstabilized and stabilized apoptotic H460 cells and necrotic cells.
Two DNA preparations were made from the control sample and 20 DNA preparations from the colonies originating from the 8 h aerobic exposure.
Histology Paraffin-embedded 5 μm sections were made from ten control and diabetic myometria, and stained with both haematoxylin and eosin (H&E) to allow measurement of muscle bundles and uterine histology.
In Fluo-4 AM imaging experiments with hippocampal cultures made from littermate control mice, the application of AP5 was able to produce a significant decrease in mSCT frequency compared to baseline recorded in TTX, as had been observed previously in wild-type rat cultures.
Therefore pools were made from samples from 'Control' animals of the same sire but a mixture of time-points (24, 48, and 72 hours).
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