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No correction was made for tissue shrinkage in assessing spatial bandwidth.
No correction was made for tissue shrinkage, which should be about 90% (Karube et al., 2004).
Thus, a mix of enhanced DAPI, TxRed, FITC, and Cy5 was made for tissue detection (VCtissue) and one for epithelial detection (VCepi): (3) V C t i s s u e = I e D A P I + I e G F P + I e T x R e d + I e C y 5, V C e p i = I e T x R e d − I e G F P − I e C y 5.
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Two libraries were made for tissues.
In order to check for tissue specificity of expression, another assembly was then made for each tissue separately using the contigs created by the full data assembly as a sequence template.
For the first experiment, for qRT-PCR as for microarray, three biological replicates were made for each tissue analyzed (i.e. leaves, flowers and mature beans).
To illustrate how histological comparisons should be made for breast tissue, published results from a large, nationally funded study will be re-examined using the proposed scheme.
In a similar study [ 78], poly[bis ethyl alanato phosphazene] constructs were made, for neural tissue engineering, by dipping a glass capillary into a polymer solution and allowing the solvent to evaporate, leaving only the polymeric material.
A length-tension curve was made for each tissue strip followed by adjustment of the muscle length to the level where developed tension was 50% of the maximal tension.
Similar observations were made for human brain tissue (not shown).
Consecutive sections were made for every lung tissue block and stained with hematoxylin and eosin.
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