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We made conservative mutations that would be predicted to neutralise these charged patches, but not affect the folding of the protein.
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To limit this inherent drawback, we were careful, whenever possible, to make conservative mutations, measure effects on helicity directly by CD, and ultimately base our analysis on >30 mutations.
That is what made conservative critics most uncomfortable".
In this costing analysis, therefore, we made conservative assumptions.
Although we made as conservative mutations as possible with respect to size when altering TM dynamics, mutants should be interpreted with caution because they can also have unintended effects, including altering the TM surface and/or interface with rhomboid.
She has long made conservatives uncomfortable.
Instead, they're making conservative short-term upgrades.
This makes conservatives better bargainers than liberals.
Both V3 sequences have a length of 35 amino acids and carry only three conservative mutations.
Within these groups, variability is introduced by insertions, deletions or by conservative mutations.
The rate of radical (Dr) to conservative mutations (Dc) was not significantly different between genes or species, because most amino acid changing mutations were conservative in nature.
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