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The relationship between the cell dry weight and the optical density was established thanks to a calibration made by triplicate using spectrophotometer at 600 nm.
The reactions were made by triplicate from at least two independent cultures.
The reactions were made by triplicate from at least two independent cultures, and the cycle of threshold (Ct) was determined for each reaction.
The assays were made by triplicate.
Sensibility tests were made by triplicate for each extract.
The assays were made by triplicate; 10 cellslls in hypertonic buffer and 10 cellslls with camptotensin were used as positive controls.
Similar(54)
This resulted in volumetric data for 6 contact lenses, each imaged in triplicate, but with some random positional variability (triplicate measurements were not made by simply imaging the contact lens in the same position three times).
Each sample was amplified in triplicate and mRNA estimation was made by comparison to duplicate standard curves and normalisation to β-actin [9].
Triplicate cell counts from each suspension were made by hemocytometer.
The remaining calibrators were made by a serial two-fold dilution (20.0 ng/μL - 1,280.0 ng/μL) and tested in triplicate to determine the most effective PCR amplification conditions.
We performed PCR in triplicate for each pool of cDNA and calculated the amounts of products using a standard curve made by sequential dilutions of isolated cDNAs for β-actin and Hoxd13.
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