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The plasmid containing the CLN2 promoter was made by swapping the promoter of pSVA17 and the one of pGC04D (using BamHI and PacI sites), resulting in pGC08D.
In fact, a weakly amphipathic version (BAX-α5MS) made by swapping the position of a pair of positively charged residues with a pair of hydrophobic residues (underlined in Table 1) shows a reduced surface pressure compared to BAX-α5M.
The Golgi-tethered forms were made by swapping the appropriate region of the coding sequence into GNT-Fj [ 12].
Furthermore, studies have shown that a chimeric SFK, made by swapping the SH3 domain of Src with that of Lck (Hck-like), displays impaired autoinhibition, while swapping the SH3 domains of Src and Fyn (both Src-like) results in a fully autoinhibited kinase.
For pDUAL-TetR-2xFLAG-Stc1, stc1+ was cloned into a modified pDUAL-HFF81c vector (Matsuyama et al., 2004), made by swapping His to TetRoff (Promega), so that Stc1 was N-terminally tagged with TetRoff followed by 2xFLAG, under the nmt81 promoter.
Similar(52)
The Laws of the Game specify that all players, regardless of gender, must wear the same kit, however in September 2008 the Dutch women's team FC de Rakt made international headlines by swapping its old strip for a new one featuring short skirts and tight-fitting shirts.
Men can make the transition by swapping shorts for long pants and wearing fitted cardigans and jackets over tees.
Decisions about when a swap pays out are made by a trade group called the determinations committee of the International Swaps and Derivatives Association.
Make a vegan version by swapping the eggs for 12 ounces of firm tofu, drained and crumbled.
In 1979 he settled a civil suit brought by the Securities and Exchange Commission, without admitting or denying wrongdoing, involving a debt-for-stock swap made by a troubled company he ran.
Wife Swap, made by the independent producer RDF Media, arrived on Channel 4 in January 2003 and was an instant hit, attracting nearly 6 million viewers before the end of its first series.
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