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The differentiation medium used to induce chondrocyte differentiation of the ATDC5 cells was made by supplementing the maintenance medium with 10 μg/ml of bovine insulin (Sigma Aldrich).
5-Fluoroorotic Acid (5-FOA, Gold Biotechnology, #F-230-5) media was made by supplementing 5-FOA to a final concentration of 600 mg/L and boiling to dissolve in LB agar miller medium.
Making a strong association between a particular chemical or class of chemicals and an adverse condition in the field is sometimes difficult, yet in some instances an association can be made by supplementing field research with well-designed laboratory research.
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For years claims made by supplement producers were not regulated.
Therefore, vaccines against many critical diseases of aquatic animals may be made more effective by supplementing TLR activators which will stimulate dendritic cells.
Crafted from brown-and-white tweed work socks with red heels manufactured by the Nelson Knitting Company in Rockford, Ill., the rag dolls appeared in the early 1900's, made by women to supplement their income.
A study of nearly 500,000 people, carried out by academics from the University of Warwick and the Johns Hopkins School of Medicine in Baltimore, USA, has delivered a damning verdict on the claims made by the vitamin supplement industry.
The qualitative research method and comments made by the mothers supplement and augment our quantitative study data.
Although some research suggests that some supplements can produce positive health effects, there have also been cases where people have been made ill by supplements, or their conditions have become worse while they tried to use ineffective dietary supplements to treat a medical disease.
Initial cross-sectional diagnoses made by sector consultant psychiatrists were supplemented by longitudinal data with renewed consultant and community mental health team diagnostic input.
Another way to verify claims made by CIs would be to supplement publicly available information with third party interviews of end-users.
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CEO of Professional Science Editing for Scientists @ prosciediting.com