Sentence examples for made by insertion from inspiring English sources

The Luk-PV plasmid was made by insertion of the luk-PV operon onto the L. lactis vector pOri23 [33].

PmUra414 was made by insertion of a c-myc tagged PCR product from the URA3 gene into a version of pRS414 bearing the TPI1 promoter.

The pBD-GmPHDs and pAD-GmPHDs were made by insertion of the GmPHD coding region into pBD vector or pAD vector.

We showed that a RGRR furin cleavage motif made by insertion of an Arg residue into the EWI-2 sequence allows efficient production of EWI-2wint and additional cleavage product(s) in Huh-7 cells.

Recombinant vaccinia constructs encoding HCMV antigens IE-1 (Vacc.IE-1), gB (Vacc.gB) and a negative control vaccinia virus construct made by insertion of the pSC11 vector alone, which is negative for thymidine kinase (Vacc.TK−), have been previously described [13].

It was notable that the cells that were left in the tract through the cortex made by insertion of the cannula did not generate cortical neurons, which suggests this might not be the case, although the environment within the tract could have been altered by other factors, such as inflammation.

To give an example of genome specific values of Sn and Sp, we provide Supplementary Table S1 for the set of 400 nt fragments with 20% containing FSs. Results are averaged between sets of fragments with FS made by insertions and FS made by deletions (see also Fig. 1).

The final readout of the microarray was made by the insertion of the slide array into a reader.

The pcDNA-KChIP3 construct was made by the insertion of the KChIP3 sequence into the pcDNA3.1 vector (Invitrogen), following amplification from KChIP3 EYFP with the following primers: sense, 5′-TTC GAATTCAGGATGCAGCCGGCTAAGGAA-3′ (EcoRI) and antisense, 5′-CAAC GCGGCCGCTAGATGACATTCTCAAACAGC-3′ (NotI).

This work helps understand the physical origin behind the remarkable tensile actuation behavior of the twisted-then-coiled polymeric artificial muscles and also provides inspirations for optimal design of advanced artificial muscles made by twist-insertion procedure.

UAS-cut-HA and UAS-cut truncation-HA lines were made by cDNA insertions into the KpnI site of pUASt-attB and targeted to the attP2 locus via FC31 integrase stocks.