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Kefir was made by inoculating and fermenting milk with kefir grains.
First, we established a novel osteolytic bone invasion model of NCI-H292 cells which was made by inoculating cancer cells into the tibia of scid mice.
Seed cultures of K. pneumoniae were made by inoculating frozen stocks made with 20% glycerol into 3 mL of glucose M9 minimal media and incubating at 37°C.
Isolation and identification was made by inoculating the selectively enriched sample on to Xylose Lysine Deoxycholate agar followed by confirmation of presumptive colonies using different biochemical tests.
Heterotypic spheroids were made by inoculating equal numbers of two ES cell types, where one population was fluorescence-labeled and was either undifferentiated or prior differentiated.
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For mutant screening, a copy of the mutant plates was made by replica inoculating the frozen stocks into 96-well plates containing 150 μl of LB medium and incubating them at 30°C for 12-18 hours with intermittent shaking.
An intermediate step for cell proliferation was made by growing the cells in 50 mL of either Glc-N-high or Xyl-N-high by inoculating with the washed cells such that initial OD620 nm corresponding to 0.05 was achieved.
To generate experimental plants, inoculum made by grinding infected leaf tissue into 10 mM KHPO4, pH 7 (1:4 w/v), was mechanically inoculated onto 1.5-week-old soybean plants.
Strain Pseudoalteromonas sp. AN178 was inoculated in the 2216E medium (peptone 0.5%, yeast extract 0.1%, pH 7.5, made by natural sea water) with shaking at 120 rpm and 8°C.
By inoculating Spain with a bailout, European financial leaders were trying to limit the possible fallout.
The Austrian psychiatrist Julius wonner-Jauregg won the Nobel Prize in 1927 for his revolutionary therapy of syphilitic general paresis by inoculating malaria.
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