Sentence examples for made blunt by from inspiring English sources

The pUAST vector was linearized by digestion with HindIII and the ends were made blunt by Klenow fragment treatment.

The ends of the DNA fragments were made blunt by incubation, in the presence of dNTPs, with T4 DNA Polymerase and Klenow fragment.

The ends of both fragments were made blunt by filling in with Klenow polymerase, and the pNBL2 backbone was treated with phosphatase.

Both ends of the excised mCherry sequence were made blunt by Klenow fragment (New England BioLabs, Ipswich, MA) treatment and the 3' end was made sticky by digestion with XhoI.

The isolated BAC DNA was nebulized and the DNA ends were made blunt by filling with T4 polymerase.

The 2690 bp EGFP-Luciferase-pA restriction fragment was then removed from pEGFPLuc at NheI and MluI sites and made blunt by a Klenow fill-in reaction.

The pShuttle(i/PPT-Luc) plasmid [19] was digested with XbaI (unique site directly downstream of the luciferase stop codon), made blunt end by Klenow fragment, followed by insertion of the HpaI/SmaI-excised miR122 target sequences (six copies) to construct pShuttle(i/PPT-Luc-miR122).

Especially as many of the advantages of keeping a sports car engine in a state-of-the-art car chassis are made blunt and brutal and less enjoyable by bolting it to an unsophisticated truck body and suspension.

I always made blunt sexual advances towards girls back then.

The ends of the fragments were made blunt using Klenow.

In order to generate a construct that would function in S. aureus, plasmid TS1 was digested with the restriction endonuclease BamHI (NEB) and subsequently made blunt-ended by treatment with Klenow fragment (Gibco).