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A 2-bp mutation (AA to CC) was made at one site on the p-full construct using the QuickChange site-directed mutagenesis kit (Stratagene, La Jolla, CA, USA).
Furthermore, decisions made at each level are interdependent since a decision made at one level can impact on the other levels.
Infrastructure decisions and transport policies are often based on the notion that all decisions are made at one single point in time, based on full information and an accurate evaluation of the benefits and costs of different alternatives.
The spot centered on the pledges made, at one point or another, by each remaining presidential candidates to shut the loophole down.
Though the recall includes all 15-inch Radial ATX and Radial ATX II tires, the focus is on Wilderness AT tires made at one Illinois plant.
Others honed in on a remark Romney made at one rally where he argued consumers should have more "choice" in selecting their health insurers.
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As it was, salvation from its foes lay only in union, and, after abortive attempts had been made at one-man rule, Saul became king of all of Israel (c. 1020 bce).
Because the acceleration rate in this process becomes 1,000 times as high as the rates produced by conventional devices, accelerators could theoretically be made at one-thousandth their current sizes.
Most buildings, cars and other props are built at one-twenty-fifth scale, although some models are made at one-twelfth scale, including the "maser tank" used to attack monsters.
Made at one-tenth of the cost of similar programs, it is now used in eight regions across the globe.
Compensation for all qualifying overtime hours (over 40) in a workweek is made at one-and-one half-times the staff member's base hourly rate.
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