Sentence examples for made assay from inspiring English sources
Three tSNPs were selected through HapMap for the IL6 gene −573 G/C (rs1800795) and −174G/C (rs1800796) in the promoter were genotyped as previously described [7], and 3437C/G (rs2069840) in the 3rd intron (Figure 1B, top panel) was genotyped by Taqman with a custom made assay purchased from Applied Biosystems ABII).
The scientific literature now includes an impressive number of 'home made" assays for various viral agents.
The inhibitors are then improved upon by a trial-and-error process in which structural modifications are made, assayed for binding affinity, and compared.
The solution was stored at 4°C covered in foil and used up to one week to make assay solutions.
To make assay plates with OP50, we concentrated a liquid culture of OP50 six times, spread 20 µl over their surface, and dried the plates for 15 minutes.
The requirement of other platforms for either surface immobilization and/or labeling steps makes assay development and species validation arduous, slow, and expensive.
A very practical consideration is that imaging can assess receptor expression at sites that are challenging to sample and assay, for example, bone metastases, where de-calcification can make assay of tumor gene products challenging.
LNAs make assay design nearly independent of miRNA GC content and compensates for many of the compromises one otherwise would have to make with a short miRNA target sequence of just ~22 nucleotides.
Unfortunately, technical and other considerations make assaying at more frequent time points impractical.
The presence of mature, uninfected DCs in infected cultures obviously makes assays that do not distinguish between latent infection and no infection very hard to interpret.
