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We made additional mutants, and the behavior of these new mutants provides additional support for the model.
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To further define the DNA binding sites on SerRS, we made additional deletion mutants of SerRS.
To address this question, we made additional Tau* mutants: Tau*C291H and Tau*C2H (C291H and C322H).
The authors could also make additional Cys mutants in the extracellular juxtamembrane region, since this region is likely to be a helical extension of the TMD helix, and moving the Cys one position at a time around the helical wheel would be expected to twist the cytoplasmic domains with respect to one another and determine which S-S dimers have activity.
Shrinkage and temperature made additional problems.
Big has, however, made additional cameo appearances.
P.K. made additional statistical analyses.
Some guidelines made additional recommendations.
To address this question, we made several additional mutants containing specific targeted mutations.
Since the high constitutive activity of the 5-HT4-D100A mutant causignificantcant phenotypes in transgenic mice [30] and could not be controlled by inverse agonists [31], we attempted to lower the Rs1 constitutive activity by making additional point mutations.
Make additional stools.
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