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Next, we made a transgenic fly in which expression of APPL conjugated with the C-terminal FLAG tag could be induced by the hsp70 heat shock promoter.
While studying the localization mechanisms of APPL and HIG, we made a transgenic fly with an insertion of a P-element containing the HIG minigene in the third chromosome.
We also made a transgenic line expressing Pn GFP fusion protein under the control of hsp70 promoter (hs-pn).
We made a transgenic mouse that expressed an N-terminally truncated form of Gli2 under the control of the lck promoter.
Reviewer #1: In this manuscript, Jansen et al. made a transgenic mouse line expressing a bacterial photoactivated adenylyl cyclase (bPAC) in testis and sperm.
To investigate the role of this gene in breast cancer, we made a transgenic model in which Runx2 is specifically expressed in murine mammary epithelium under the control of the mouse mammary tumour virus (MMTV) promoter.
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Moreover, when a drug maker needs to double production, it simply breeds more animals thereby avoiding spending $300m on a new factory.Mr Newberry reckons it takes about 18 months to make a transgenic goat that produces a desired therapeutic protein in its milk.
(Making a transgenic mountain vole would have been much more difficult).
"If you make a transgenic plant that produces that alarm continuously, it's not going to work," he says.
The simplest method of making a transgenic animal is to use a single DNA construct in which a promoter directly regulates the expression of a transgene.
We did this by making a transgenic mosquito line that carries an Enhanced Green Fluorescence Protein (EGFP) fused to the AAEL0010824 promoter that we defined here.
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CEO of Professional Science Editing for Scientists @ prosciediting.com