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These techniques range from traditional "macroscopic" methods such as batch and flow-through reactors [7, 15, 17], to surface sensitive techniques [13, 18 22] to studies at the "nanoscale" performed by means of Atomic Force Microscopy [11, 23 29].
Although this technique is macroscopic, contrary to other macroscopic methods such as thermogravimetrical and chromatographic techniques, the diffusivities obtained are in agreement with those determined using microscopic techniques such as NMR.
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Furthermore, this macroscopic method is the first technique that yields diffusivity values which are in good agreement with those determined using microscopic methods, such as Pulse-Field-Gradient NMR.
Some of the best known macroscopic image analysis methods such as imaging with ionizing radiation, magnetic resonance imaging, and ultrasound imaging are described in the chapter.
In this regard, the establishment of standardized procedures [ 39] for the evaluation of gait parameters in murine OA models should be added to existing methods, such as whole joint macroscopic pathology ([ 22] and present study), confocal laser-scanning microscopy [ 40] and histopathology [ 41].
Invasive methods such as colonoscopies and biopsies have shown macroscopic and microscopic gut inflammation in 25 to 60% of AS patients while noninvasive observational studies report clinical overt IBD in 5 to 10% of AS patients (as reviewed in [ 35]).
However, they are not readily diagnosed with standard diagnostic methods such as MRI since they are without macroscopic evidence of structural damage, defined as absence of fibre tear on MRI.
Furthermore, our present method to confirm blood supply of the remnant pancreas and spleen by macroscopic findings is unreliable and not objective, and therefore much more secure methods such as color Doppler ultrasound and indocyanine green fluorescence angiography [ 27] should be introduced in the future.
Application of novel analytical and investigative methods such as fluorescence in situ hybridization, confocal laser scanning microscopy (CLSM), microelectrodes and advanced numerical simulation has led to new insights into micro-and macroscopic processes in bioreactors.
Surface-sensitive and high-resolution techniques were used to complement information gathered from classical, macroscopic methods.
Macroscopic methods are especially important in epidemiological studies.
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