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Only 4T1 forms macroscopic lung and liver metastases.
In this case, all mice died with large macroscopic lung and disseminated metastasis, indicating that LVR01 alone was insufficient to control the 4T1 metastatic process.
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In the same way, polymicrobial sepsis inhibited the pulmonary metastatic spread as assessed by macroscopic lung pathology and lung infiltration onto histological slides.
The unpaired Student's t-test was used to compare antibody titers and macroscopic lung lesion scores.
It should be noted that the data from qPCR showed much stronger correlations with the microscopic and macroscopic lung lesions compared to IF suggesting that qPCR is more suitable to investigate the number of organism that IF.
The lungs were removed en-bloc after thoracotomy and a macroscopic lung injury score [ 15] was applied: four ventral and dorsal segments (each upper/lower right, upper/lower left) of the lung surface were examined for hemorrhage and congestion (2 points > 50%, 1 point for < 50%, 0 points for no or minimal changes).
These 5 calves shed high titers of virus and developed severe clinical signs of disease and extensive macroscopic lung lesions (mean+/−SD, 48.3+/−12.0% of lung), with a pulmonary influx of inflammatory cells, characterized by interferon gamma secretion and a marked effect on lung function.
4T1 cells are fully metastatic and form macroscopic lung nodules from primary mammary tumors.
All of the non-survivor animals experienced breathing difficulties before death and showed numerous macroscopic lung metastases as was previously observed in untreated mice (see Fig. 1c).
The reduction in macroscopic lung PA was statistically signficant (p<0.05) for both BCG/MVA and SO2 treatment and approached significance (0.1>p>0.05) for BCG alone.
In addition, from each patient, a small section of normal lung parenchyma distant from the macroscopic lung cancer tissue was removed at surgery and stored frozen.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com