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The lowest concentration leading to no growth (i.e. no turbidity), as assessed by macroscopic evaluation was taken as the minimum inhibitory concentration (MIC).
Macroscopic evaluation was further strengthened by computerized image analysis).
First, macroscopic evaluation was performed for tibial and femoral cartilage surfaces stained with India ink.
Development of arthritis by macroscopic evaluation was determined as described previously [ 19].
The lowest concentration, which inhibits the visible growth of tested organism after macroscopic evaluation, was determined as MIC.
Macroscopic evaluation was based on criteria reflecting inflammation, including bowel wall thickening, luminal distension and bleeding, intestinal tissue necrosis and rupture, intestinal edema, and erythema and haemorrhage.
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Furthermore, macroscopic evaluation is a subjective and therefore imprecise factor.
The lowest concentration which did not show any growth of test organism after macroscopic evaluation is defined as MIC.
Data on specimens' macroscopic evaluation were obtained for 107 individuals and was averaged for 2 5 biopsies/individual to obtain a single value per individual, which was used for the correlations and tables presented here.
Under macroscopic and microscopic evaluations, all animals of the control group were of grade 0. No significant changes in macroscopic or microscopic evaluations were found in the control group.
Standardized neuropathologic assessments, including macroscopic and microscopic evaluations, were carried out with assignment of a pathologic diagnosis using established DLB criteria [ 13, 25].
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