Sentence examples for macrophages as described from inspiring English sources

All experiments were conducted between days 10 and 12. Isolated monocytes were seeded in the lower chamber of a six-well transwell apparatus with 0.4-μm pore size (Corning, Lowell, MA, USA) and differentiated to macrophages as described above.

We then tested the differential expression of genes between macrophages derived from blood monocytes or from tissue resident embryonically-derived, self-renewable macrophages as described by Geissmann and Lambrecht teams22,23.

Immunohistochemical staining with the mouse macrophage specific antibody (Mac-3, clone m3/84, BD Pharmingen, BD550292, USA - BD Biosciences Pharmingen, Franklin Lakes, NJ, USA) was performed using aortic longitudinal 8- μm cryosections in order to detect activated macrophages, as described earlier [10].

T cells were then incubated with BCG infected macrophages as described below.

Peripheral blood monocytes were prepared from human blood as previously described [66] and were differentiated to macrophages as described [33].

After 12 h of infection, the intracellular bacteria were isolated from the infected macrophages as described previously [30], [36].

Human monocytic cell line THP-1 (ATCC) cells were cultured and differentiated into macrophage as described.

Tumour bearing CB-17/lcr SCID mice were depleted of effector cells by using anti-asialo-GM 1 (1.25 mg kg−1, neutrophils) and anti-Gr-1 (4 mg kg−1, NK cells) clodronate encapsuled liposomes (CEL, macrophage), as described earlier (Oflazoglu et al, 2007).

RAW264 macrophage cell lines (ATCC) and immortalized macrophages (generated as described below) were cultured in RPMI 1640 (same supplements as above).

RNA was prepared from exponential cultures of M. tuberculosis strains grown in vitro or in macrophages essentially as described [51].

Isolation of mouse peritoneal macrophages was as described.