Exact(4)
Terminal ApaI macro restriction fragments were isolated by digesting PFGE-resolved full-length chromosomes with BssHII restriction endonuclease.
Sma I Macro Restriction Profiles, Sequence Types and antimicrobial resistance profiles of ovine and bovine C.jejuni abortion-associated isolates.
Strain typing of CRKP was done by comparison of XbaI macro restriction profiles generated by pulsed field gel electrophoresis (PFGE) according to the criteria of Tenover [ 9].
Polaroid photographs of macro restriction profiles were scanned with a UMAX Vista-S6E scanner (UMAX Technologies Inc, Dallas, Texas, USA) and digitalised using Adobe Photoshop 3.0.5 for Windows, and saved in TIFF format.
Similar(56)
Macro-restriction analysis of the isolates was performed at SVA.
The relatedness of isolates was determined through a combination of phage typing and macro-restriction pulsed-field gel electrophoresis (PFGE).
Analysis of SmaI macro-restriction profiles of the 16 MRSA isolates revealed one dominant clone (14 isolates).
To improve pulsed-field gel electrophoresis based strain discrimination of 76 Salmonella Enteritidis strains, we evaluated 6 macro-restriction endonucleases, separately and in various combinations.
Pulsed-field gel electrophoresis (PFGE) with MluI restriction enzyme was used to compare DNA macro-restriction profiles between isolated spirochaetes and the challenge strains, as described earlier [ 11].
Macro-restriction analysis by PFGE was also carried out by SVA, using the enzymes Cfr9I, a neoschizomer of SmaI, and ApaI according to the HARMONY protocol [ 40].
Further analysis through phage typing and macro-restriction pulsed-field gel electrophoresis indicated that wild passerine deaths associated with salmonellosis were caused by closely-related S. Typhimurium isolates, some of which were clonal.
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