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Mammalian mitochondria have their own NAD biosynthetic machinery which plays a key role in maintaining mitochondrial NAD pool [13, 115].
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Proteins, the main cell machinery which play a major role in nearly every cellular process, have always been a central focus in biology.
In the present study, we used siRNAs directly targeting UPF1 which plays a central role in the NMD machinery.
The 3D protein is the viral RNA-dependent RNA polymerase (RdRp), which plays an essential role in the replication machinery of RNA viruses 22, 23.
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These non-coding transcripts are processed by the RNAi machinery to form siRNAs which play an important role in the assembly of heterochromatin at the centromere.
Signal features are biologically functional regions including core-promoter elements [ 11, 12], some short modular transcription factor binding sites (TFBSs), and CpG-islands [ 13], which play important roles in assembly of transcriptional machinery.
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With our proteomic approach we were able to demonstrate consequences of glucose limitation for the protein expression machinery which also could play a role for a higher recombinant protein production.
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