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Although beneficial properties may emerge from such a broad range of perturbations on cellular machinery, validation and/or prediction of their activity profiles is accompanied with a variety of difficulties in generic risk-benefit assessments.
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What will validation validate?
In addition, the uncertainties associated with this analytical approach can be compensated by a stepwise implementation of the machinery combined with an on-site validation of the relevant design parameters.
However, identification and functional validation of chromatin-interacting RNAs are technically challenging with respect to distinguishing RNAs that bind chromatin modifying machinery from artifacts.
Among the former, 9 of the miR-107 potential target genes were selected for further validation in our cohort, including genes involved in the PI3K/Akt signaling pathway and in the RNA-interference processing machinery.
Machinery malfunctioned.
There as machinery.
His machinery is decrepit.
Machinery wears out.
Hence the heavy machinery.
Take out the machinery".
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