You have to have characters interacting with something a situation, or possibly a piece of machinery (such as a colonoscope, for instance), or with each other.
But as soon as you can set up an intracellular molecular transport machinery such as a filamentous cytoskeleton and associated molecular motors, then having the genome be readily accessible to diffusive transport becomes less of an issue, freeing up eukaroytic cells to become physically large.
When expressed during early stages of DNA replication and cell division, GcrA up-regulates transcription of 89 C. crescentus genes, including several components of the cell's DNA replication machinery such as a DNA gyrase, topoisomerase IV and a DNA pol III epsilon subunit [ 89].
However, the small quantities of complex I proteins observed by Juszczuk and Rychter (2009) could be due to defective translational machinery, such as a compromised small ribosomal subunit, rather than a defective or under-represented protein in this complex as may be the case for MSC3.
Pirsig said its protagonist "set out to resolve the conflict between classic values that create machinery, such as a motorcycle, and romantic values, such as experiencing the beauty of a country road".
Mr. Unger, the Brazilian minister, agreed, saying that the BRIC countries do not see replacing the dollar with "heavy-handed, bureaucratic machinery," such as a global, European-style Central Bank.
This usually requires heavy machinery such as a mill.
The wheel type includes typical construction machinery such as a truck.
In addition, some members of other groups also appear to have lost their catalytic machinery, such as a Xanthomonas-specific subgroup that appears to rapidly emerge from subfamily GH5_1 (Additional file 1: Figure S1).
Use all safety protection when operating machinery (such as a circular saw!).
