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One limb, termed the fusion machinery limb, contains Rab3A, PTP1B, α-SNAP/NSF, and SNAREs.
Once the system has progressed beyond the loop, the calcium mobilization limb will proceed regardless of the fusion machinery limb.
In other words, low anti-Epac antibody concentrations would impair only the calcium mobilization limb whereas higher concentrations would interfere with calcium mobilization as well as with the fusion machinery limb.
We could not anticipate the results because we knew that blocking Rab3 affected the other branch but blocking SNARE complex assembly did not (Rab3 is upstream of PTP1B and SNARE complex assembly is downstream of this phosphatase, all three are in the protein machinery limb, Figure 1).
To answer the question posed before about how far after Rab3 can we go before the two signaling pathways become independent we forced the protein machinery limb to move downstream of Rab3 with the reversible pair substrate trapping mutant PTP1BD181A/wild type PTP1B.
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Soderbergh was looking for a setting that would ignite his imagination, and the world of plastic limbs, whirring machinery and shiny eyeballs sounded a good bet.
Then all day long I have been juggling phone calls and schedules and collecting masses of free paper from Westferry newspaper printers, all limb-mangling machinery and false forests of print paper.
Accidents were so common an ax was kept handy to slice off limbs caught in machinery.
The book belies the dryness of its subtitle with tales of business deals turned violent and limbs mangled in machinery.
They were makeshift affairs: a tree limb, pieces of junked machinery, concrete blocks.
The positive limb of the mammalian clock machinery is comprised of CLOCK and BMAL1, which are transcription factors that heterodimerize through the PAS domain and induce the expression of clock-controlled genes by binding to their promoters at E-boxes.
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