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We found robust induction of autophagy after starvation in both control and MLIV cells, indicating that the autophagy machinery is functional in MCOLN1-deficient cells.
Furthermore, the ability of autophagy to elicit a cell-survival or cell-death response seems to be significantly impacted by whether or not apoptotic machinery is functional.
To address whether Atg7 alone or the whole autophagic machinery is functional in the neonatal mouse ovary, we established an in vitro culture system for neonatal ovaries to mimic the in vivo neonatal transition process.
Given that the mitochondria is a key component of onset of apoptosis and ClpQY machinery is functional in the parasite mitochondria, we first studied the effect of Ant-FI-peptide treatment on mitochondria membrane potential by JC-1 dye staining.
These experiments are particularly important because, unlike evidence showing the presence of cell cycle markers in neurons, which could be dismissed as epiphenomena of suspect physiological relevance, they demonstrate that the DNA replication machinery is functional and capable of completing S phase in post-mitotic neurons.
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Even though we had detected 15S rRNA by end-point PCR in nascent non-complemented Δ ccm1 segregants, it was not clear that such levels were above the threshold at which the translational machinery was functional [ 14].
The modulation of gene expression by the microRNA-mediated silencing machinery is gaining functional importance in neurons (Kosik, 2006).
This indicates that (i) the downstream apoptosis machinery is usually functional in these cells, and, that (ii) they are ready-to-die and as such strictly dependent on niche-derived pro-survival signals.
Furthermore, expression of the ppdDhofBC (hcpABC) gene cluster from EHEC in an E. coli K12 strain resulted in surface expression of the PpdD pilin, suggesting that the T4P assembly machinery in K12 strains is functional [32].
Thus, like eS26, GFP-eS26 is functional to recruit specific components of the import machinery.
The furniture is functional, too.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com