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Interestingly, the cellular silencing machinery is active at early times post-infection and can be used to control the adenovirus cell cycle.
As the RNAi machinery is active during differentiation, the upregulation of Jmjd3 in knock-down clones at day 7 reflects most likely the selection pressure that enriches the culture for those cells with less efficient knock-down and higher levels of Jmjd3.
The ability to silence a specific gene in tick eggs suggests that RNAi machinery is active in the tick embryos.
The results reported herein extend those of Nijhof et al. [ 22], that unprocessed dsRNA in adult ticks not only can be transovarially inherited by the egg stage, but that the RNAi machinery is active in tick eggs.
Such pervasive changes in localization status of mRNAs contradict the model that mRNAs localize through sequence encoded mRNA zipcodes (reviewed in Medioni et al., 2012) and that the general localization machinery is active in all cell types analysed (Bullock and Ish-Horowicz, 2001; Jambor et al., 2014).
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Half a dozen cranes and other pieces of heavy machinery were active at the site, transporting hardware and pumping water from the pit — Bentonville had recently suffered almost two straight weeks of rain.
These results indicated that the ribosomal synthetic machinery was active in both muscles and that this up-regulation seemed to be coupled with the down-regulation of genes involved in fast-glycolytic features in the gene network conditions.
Ad 5. Accumulation of protein aggregates, as seen during MDB formation, is counteracted by proteasomal or autophagic degradation (Glickman and Ciechanover 2002; Williams et al. 2006) and both degradation machineries are active in the liver.
At a subsequent stage, once the HR machinery is fully active, the cell cycle is normally stalled by the activation of the DNA damage checkpoints.
This finding clearly indicates that the RA-mediated transduction machinery is not active in these cells shortly after their arrival in the PAs [22].
However, although our current results confirm this view, we see the glass half empty because most genes are productively transcribed just once or a few times per cell cycle and that most of the transcriptional machinery is not active at the same time.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com