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This indicated nonrandom (caused by the dysfunction of splicing machinery) changes in alternative splicing.
In particular massive transcriptional changes were induced that apparently lead to a down-regulation of the metabolic machinery, changes in the actin cytoskeleton and the up-regulation of the stress response.
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Toxicity was shown to be associated with oversaturation of the cellular RNAi machinery and changes in endogenous miRNA expression.
The in vitro studies presented here are consistent with this hypothesis; the CNDAC/vorinostat combination (Table 1 and Figure 1) targets multiple arms of the apoptotic machinery, producing changes in the levels of key proteins involved in regulating apoptosis.
Multiple regulators and core components of the autophagic machinery undergo changes in their acetylation status, and acetylation of histone lysine residues has been linked to different aspects of autophagy.
RIL should not lead to major additional investments (re-engineering of harvesting machinery and change in methods are not required).
The finding that a part the splicing machinery can change in closely related organisms can lead to some interesting hypotheses for evolutionary adaptation, that could be particularly relevant in the response to sudden and strong selective pressures.
Production and maturation of miRNAs require a set of proteins collectively known as the miRNA biogenesis machinery and it is now established that alterations in this machinery can generate changes in miRNA expression and contribute thus in the development and progression of cancer.
Studies have ranged in their approach from identifying the molecular machinery responding to changes in nutrient levels, to understanding the hormonal changes that occur in response to diet, to mapping the response of differing life history traits over complex dietary landscapes.
By "static effects" of calcium we refer to calcium-dependent mechanisms that participate in setting the operating point of a system (here phototransduction machinery) while moderate changes in the feedback signal (here [Ca2+]i) do not affect the output signal through the mechanisms of the static effect.
The molecular machinery that senses changes in the micro-environment of cells and translates them into epigenome modulations of DNA or histone tails, thereby influencing gene expression, is comprised of various kinases, acetyltransferases, and methyltransferases.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com