Sentence examples for machine kit from inspiring English sources
The human wild-type67 mRNA of RHEB (NM_005614) was cloned into the pCS2+ vector and transcribed in vitro using the SP6 Message Machine kit (Ambion).
The plasmid was linearized with ScaI and transcribed with T7 polymerase (sense RNA) or T3 polymerase (antisense RNA control) using the mMessage Machine Kit (Thermo Fisher) according to the manufacturer's instructions (Invitrogen/Thermo Fisher).
Uncropped blots of all Western blot data presented in the main figures are shown in Supplementary Figure 8. RNA was synthesized using the SP6 mMessage Machine kit (Thermo Fisher Scientific, AM1340).
As of Thursday morning, the Goldie Blox and the Spinning Machine kit was ranked eighteenth in the Toys and Games category on Amazon; more than twenty other construction and science toys, including three Lego Friends sets, number in the top hundred.
Messenger mRNA was synthesized using the mMessage Machine Kit (Ambion).
10 µg of linearized vector were in vitro transcribed with SP6 using the mMessage Machine kit (Ambion).
Synthetic capped mRNA and anti-sense RNA dig-labeled probes for in situ hybridization were prepared by using mMessage Machine Kits (Ambion).
Bodies that Picabia once depicted as planes of color now appeared as complex machines, kitted out with pumps and valves, greased by desire.
Following linearization of wild-type, XYl-excised and chimaeric PLC plasmids, cRNA was synthesized using the mMessage Machine T7 kit (Ambion) and the poly(A) tailing kit (Ambion), as per the manufacturer's instructions.
Following linearization of WT and mutant PLCζs, cRNA was synthesized using the mMessage Machine T7 kit (Ambion) and a poly(A) tailing kit (Ambion), as per the manufacturer's instructions.
After linearization of the various luciferase-tagged PLC plasmid constructs, complementary RNA (cRNA) encoding the respective PLC was synthesized (13, 14) by use of the mMessage Machine T7 kit (Ambion) and a poly(A) tailing kit (Ambion), as per the manufacturer's instructions.
