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A group of five rhesus macaques were immunized at weeks 0 and 4 with 1 mg/construct of pH5HA, pNP, and pN1NA.
Macaques were immunized at weeks 0, 8, and 16 using a disposable single-shot particle-mediated delivery device designed for clinical use that delivers plasmid DNA directly into cells of the epidermis.
Five Rhesus macaques were immunized i.m. with 5 mg of the hLAMP/gag plasmid at weeks 0, 4, 14, 22 and 38, and the cellular immune responses of each of the individual macaques to the hLAMP/Gag chimera were measured at 12 time points, through 42 weeks (Figure 3).
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The procedure of immunization, library cloning, selection of antibodies and screening is given in figure 4. A male macaque was immunized with recombinant Crf2.
Effective boosting of both Gag-specific CD8 and CD4 T cell responses was observed at one week following the second immunization (1st booster immunization) in both groups of immunized macaques, but was significantly more pronounced (group median responses were approximately 2-fold higher) in those macaques that were immunized with the MVAΔudg-gag vector as compared to MVA-gag (Figure 9A, B, D).
We used 12 cynomolgus macaques, of which 10 were immunized with VSVΔG/ZEBOVGP either orally (OR; n = 4), intranasally (IN; n = 4) or intramuscularly (IM; n = 2).
Adult macaques (2.5 4 years old) were immunized i.n. with 7.8 lof10 TCID50/animal of virus without narcosis in order to limit virus application only to the upper part of the respiratory tract mimicking immunization in humans.
EC50 values were calculated by nonlinear regression analysis of the replicate dilution series for each sample using Prism 4 software (GraphPad Software, Inc .. Male rhesus macaques, aged 3.5 5 years were immunized with a total of 2×108 PFU of MVAΔudg-gag or MVA-gag that was delivered by a split route: 1×108 PFU intradermally and 1×108 PFU intramuscularly into alternate thighs.
When they were ten, they were immunized against polio.
Female C57BL/6 mice were immunized with MOG35 55.
Mice were immunized on days 0, 7, 14, and 21, respectively.
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