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At the same time, 28 other macaques were exposed to the virus but not the vaccine.
Within a negative-pressure (−24.9 Pa), head-only aerosol exposure chamber, macaques were exposed to a small-particle (0.5 3 μm aerodynamic diameter targeting lung alveoli) aerosol challenge (inhaled dose = log10 – 4.64 plaque-forming units).
To characterize the pathogenesis in cynomolgus macaques (Macaca fascicularis), groups of macaques were exposed to four different doses of aerosolized MPXV.
In this study, four groups of cynomolgus macaques were exposed to increasing doses of aerosolized MPXV, with a particle size of 1 3 µm (Table 1).
Cynomolgus macaques were exposed to target concentrations of 0, 2, or 6 ppm formaldehyde.
To study the effects of formaldehyde inhalation exposure, cynomolgus macaques were exposed to approximately 0, 2, or 6 ppm formaldehyde 6 hr/day for 2 days.
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Subsequently, each macaque was exposed to MPXV in a head-only chamber contained in a class III biological safety cabinet located inside a BSL-3 suite.
This is consistent with its therapeutic effect and with previous findings that sertraline, another SSRI, ameliorated anxiety-related behaviors and alcohol abuse in rhesus macaques that were exposed to repeated separation stress [17].
Nine anthrax-naïve rhesus macaque NHPs were exposed to approximately 8 LD50 of B. anthracis spores (Ames strain) by a head-only aerosol exposure system.
Six rhesus macaques (RM) of Indian origin were exposed intravaginally to various dilutions of a SHIV-1157ipd3N4 (R5-SHIV) stock grown in RM peripheral blood mononuclear cells (PBMC) (227 ng/ml of p24; 4×106 /ml) 50% tissue culture infectious doses (TCID50) as titrated in TZM-bl cells.
Human and macaque blood samples maintained on ice were exposed ex vivo to 0.2, 0.6, 1 and 2 Gy doses of 137Cs γ-rays (dose rate: 0.69 Gy min−1) in a Mark-1 γ-irradiator (JL Sherpherd & Associates, San Fernando, CA).
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macaques were assessed
macaques were observed
macaques were fasted
macaques were prepared
macaques were treated
macaques were supplied
macaques were inoculated
macaques were acquired
macaques were determined
macaques were implanted
macaques were scored
macaques were infected
macaques were elevated
macaques were divided
macaques were left
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