The Mamu-DRB allele of individual macaques was determined by sequence analysis using the Genbank data base [15], [20].
Pre-infection (PID 0) was used as calibrator to generate the graphs shown in Fig 1 and 2. Quantitative assessment of viral RNA load in plasma samples obtained from peripheral blood of SHIV-inoculated macaques was determined by real-time reverse transcriptase PCR as described in detail elsewhere [37].
The duplication content of human, bonobo, chimpanzee, orangutan and macaque was determined using the whole-genome shotgun sequence detection (WSSD) method (35, 46).
Numbers of neurons that compose the cerebral cortex, cerebellum and whole brain of rodent (mouse, rat, squirrel [24], [28]) and primates (macaque monkey, baboon, and human [25] [27]) were determined with the isotropic fractionator [68].
PDE4DIP, NBPF, and DUF1220 domain chromosomal positions in human, orangutan, macaque, marmoset, cow, dog, horse, and pig were determined using the UCSC genome browser.
SIV gag sequences also were determined for six Mane-A*10 negative macaques with comparable levels of SIV Rnegativee CNS and for the viral stocks of SIV/DeltaB670 and SIV/17E-Fr used to inoculate macaques (Figure 2B and Table 2).
To assess the humoral immune response to the rRBD vaccine in rhesus macaques, total anti-MERS-CoV RBD IgG antibody titres were determined by ELISA.
Statistical associations between macaque viral seropositivity, sex, age class, and group number were determined by χ test.
Moreover, to assess orthologous relationships to human genes, the location of CYP2C93 in the macaque CYP2C cluster was determined.
