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The requirement for HTLV-1 infectivity of orf-I is demonstrated by the loss of virus infectivity in macaques exposed to an engineered virus, whereby expression of orf-I was ablated.
Overall, cynomolgus macaques exposed to aerosolized MPXV develop a clinical disease that resembles that of human monkeypox.
The bonnet macaques exposed to repeated social separation stress displayed increases in anhedonia that involved a cluster of symptoms typically seen in depressive monkeys [33], including macaques [34].
Our results show that bonnet macaques exposed to repeated social separation stress display depression-like features associated with suppression of hippocampal neurogenesis.
In the two previous studies related to neurogenesis in primates, hippocampal neurogenesis was suppressed in both adult marmoset monkeys exposed to acute intruder stress [14] and in juvenile rhesus macaques exposed to acute prenatal stress [15].
We present that cynomolgus macaques exposed to aerosolized MPXV show many characteristics of monkeypox and smallpox in humans and is thus an appropriate model for orthopoxvirus pathogenesis, vaccine and therapeutic studies.
Similar(41)
We verified this protocol using archived plasma from one rhesus macaque exposed to aerosolized Ebola virus.
To characterize the pathogenesis in cynomolgus macaques (Macaca fascicularis), groups of macaques were exposed to four different doses of aerosolized MPXV.
Virus-specific Gag 181 CD8+ T-cell response in the blood of macaques exposed intravenously to SIVmac251 appears to peak within the first weeks following intravenous exposure [ 40].
At the same time, 28 other macaques were exposed to the virus but not the vaccine.
Within a negative-pressure (−24.9 Pa), head-only aerosol exposure chamber, macaques were exposed to a small-particle (0.5 3 μm aerodynamic diameter targeting lung alveoli) aerosol challenge (inhaled dose = log10 – 4.64 plaque-forming units).
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