When we inoculated macaques with lower doses (data not shown) or when macaques were inoculated on multiple occasions, incubation periods were highly variable.
VACV GP-inoculated macaques were challenged 45 days after the third vaccine dose, EBOV liposome-vaccinated animals 35 days after the third vaccine dose, and macaques vaccinated with the gamma-irradiated whole-virion preparation 43 days after the third vaccine dose.
To investigate gastrointestinal absorption of intact gluten peptides, we dosed a gluten-sensitive macaque with an isotopically labeled form of the immunotoxic 33-mer gluten peptide [13], and measured its plasma concentration using a sensitive and specific mass spectrometric method.
Unlike the response to HAV and hepatitis B virus, the response to HEV in macaques is very dependent upon the dose, with little evidence of hepatitis from doses below 103.0 infectious doses, administered intravenously.
Immune sera were generated by injecting rhesus macaques intramuscularly with a single dose of 10, 20 or 100 μg of either CP5 (CP5- CRM197) or CP8 (Cpolysaccharidelysaconjugateconjugate vaccine where the polysaccharide was chemically conjugated to the detoxified diptheria toxin CRM197.
Two groups of macaques received two doses of the corresponding NYVAC-C-KC vectors (weeks 0 and 4) and booster doses with NYVAC-C-KC vectors plus the clade C HIV-1 gproteinotein (weeks 12 and 24).
Three cynomologus macaques were vaccinated with 1 dose of 10 PFU of VSVΔG/LASVGPC, a live-attenuated, recombinant viral vaccine in which the VSV surface glycoprotein has been replaced with those of LASV, by intramuscular injection as described (4 ).
It is interesting to note that a similar dose response was recently reported in rhesus macaques immunised with a similar DNA vaccine coding for human PSA (Kim et al, 2001).
Briefly, cynomolgus macaques were infected with a low dose (roughly 25 CFU/macaque), virulent strain (Mtb Erdman) by bronchoscopy.
doses of the TLR3 agonist poly (ICLC) delayed the viremia in rhesus macaques infected with YF [30] and eliminated or delayed the viremia in animals challenged with VEE virus [31].
