Exact(30)
In a manner similar to Fab 1, native Mab 1 was also inhibitory but to a lesser extent (60 30%).
A suboptimal concentration of anti-CD40 mAb (1 µg/ml) did not affect Beclin 1 expression and did not induce anti-T.
Anti-E-tag mAb (1 mg, Phadia) was previously crosslinked to protein G-Sepharose resin (1 ml, Sigma) with dimethyl pimelimidate dihydrochloride (DMP; Sigma).
The wells were washed with PBS and treated with biotinylated anti-IFN-γ mAb (1 µl/ml; 100 µl/well) and incubated at 37°C for 3 hours.
Whole splenocytes from WT or Nur77Tg mice were stimulated for 72 h with soluble CD3 mAb (1 µg/ml) alone or CD3 plus CD28 mAbs (0.5 µg/ml).
In short term cultures PBMC were activated for 4.5 h by PMA (50 ng/ml) and ionomycin (1 µM) or for 24 hours in flat-bottom 96-well plates in presence of coated anti-CD3 mAb (1 µg/ml) and soluble anti-CD28 mAb (1 µg/ml).
Similar(30)
Antibodies: mouse anti-rat ED1 monoclonal antibody (mAb, 1∶200, clone OBT1150, Immunological direct), rat anti-mouse CD11b mAb (1∶500, clone M1/70, BD Biosciences).
The primary antibodies included mouse anti-DHFR mAb (1∶250, BD Bioscience), mouse anti-p53 mAb (1∶1000, DO-1), mouse anti-p21 mAb (1∶1000, F-5), and mouse anti-α-tubulin mAb (1∶1000, TU-02) from Santa Cruz Biotechnology.
IL-33 mAb (1/200, clone Nessy-1) and polyclonal antibodies (1/200, Cter1 and Cter2, nos 210-447 and 210-933) were obtained from Alexis Biochemicals.
Protein expression was detected by the rabbit anti-GFP Ab (1∶300, Molecular Probes) and MB lobes were labeled by the 1D4 mAb (1∶80).
Antibodies employed for Western blot were: anti-E-tag mAb conjugated to peroxidase (POD) (1∶5000; GE Amersham Biosciences); anti-E-tag mAb (Phadia; 0.5 mg/ml), anti-GroEL mAb-POD (1∶5000; Sigma); anti-β-lactamase mAb (1∶1000; QED Bioscience); anti-GFP mAb (1∶1000; Roche); anti-GAPDH (1∶2000; Santa Cruz Biotechnology); anti-DnaK (1∶5000; Stressgen).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com