To identify materials on airplanes that were likely to contain PBDEs, we used a portable XRF analyzer (Innov-X, Woburn, MA) to quantify bromine concentrations in materials aboard the 19 airplanes.
All animals underwent IVIS imaging of their forepaws at regular intervals by placing the animal in a light-tight chamber, and images were generated over a 10 s exposure using a cryogenically cooled charge-coupled camera (IVIS Lumina II, Caliper Life Sciences, Hopkinton, MA) to quantify photons emitted by the animal.
An ELISA kit (BTI, Stoughton, MA) was used to quantify the purified rHLZ, in accordance with the manufacturer's instructions.
An ELISA specific for hASM (Genzyme, Framingham, MA) was used to quantify the level of the enzyme in serum samples as reported [9].
A whole body dual energy X-ray absorptiometry (DXA) scanner (Hologic QDR 4500, Bedford, MA) was used to quantify participant's total body fat mass.
Dual-energy X-ray absorptiometry (Hologic, Bedford, MA) was used to quantify fat mass (kilograms), fat-free mass (kilograms), and percent body fat.
Dual-energy X-ray absorptiometry (Hologic, Bedford, MA) was used to quantify percent body fat, total fat mass and fat free mass.
The Human IL-1ra/IL-1F3 Immunoassay (R&D Systems) and the MMP-13 Human ELISA Kit (Abcam, Cambridge, MA) were used to quantify IL-1Ra and MMP-13 protein levels, respectively, in PHCs 24 hours following treatment with MATN3 and/or IL-1β, according to the manufacturer's instructions.
Videos were later analysed using in-house ImageJ (NIH, Bethesda, MA, USA) stack tools to quantify rolling velocities.
The DELFIA TRF (dissociation-enhanced lanthanide fluorescent immunoassay with time-resolved fluorescence) assay platform (PerkinElmer, Waltham, MA, USA) was used to quantify secreted IL-8.
A membrane invasion culture system (Collaborative Biomedical Products, Bedford, MA, USA) was used to quantify cell invasion and migration, as described previously (Rozic et al, 2001).
