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The genomic profiles selected were mutations, putative copy-number alterations from GISTIC and mRNA expression data (mRNA Expression z-Scores (RNA Seq V2 RSEM)).
Data sets 2A and B summarize all mRNA expression data.
All mRNA expression data of human samples have been generated by Affymetrix U133plus2.0 arrays.
(a) Lung cancer cell lines (columns) were clustered based on a combination of PTMs and mRNA expression data (rows).
The mRNA expression data convincingly showed the expression of INS gene in all 16 measurements (preadipocytes and adipocytes of both subcutaneous and visceral cells, first experiment).
Tumor mRNA expression data and linked clinical data sets were from TCGA,45 a publically available HiSeq RNA sequencing gene expression profiling data sets of human CRC samples.
Using cBioPortal (www.cbioportal.org), mRNA expression data for TBX3 and TBX2 in sarcoma patients (TCGA, Provisional, 265) were visualized on a heatmap and clustered.
The mRNA expression data and clinical information of AML samples were obtained from a patient cohort reported by Verhaak and colleagues22.
(a) Gene set enrichment analysis (GSEA) of mRNA expression data of PC3 organoids at day 5 or 8 in 3D culture.
Nrf2 siRNA reduced significantly Nrf2 mRNA expression (data not shown).
Immunohistochemistry confirmed mRNA expression data enabled mapping of protein expression.
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