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Voltage and current electrodes (0.4 2 M) were filled with 3 M KCl.
Three identical belts (length: 33 m, width: 3 m, and depth: 0.5 m) were filled with stone (bottom), gravel, and mangrove sand (surface).
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A circular pool (ø 1.2 m) was filled with water which was painted white with non-toxic paint and kept at a temperature of 25°C.
The watermaze (diameter 2.0 m) was filled with water to a depth of 45 cm containing 500 mL of white poster paint to render the water opaque.
A large circular tank (diameter 1.2 m, depth 0.4 m) was filled with opaque water (25 ± 1°C, depth 0.3 m) and the escape platform (10 × 10 cm) was submerged 1 cm below the surface.
Six adjacent concrete raised beds (each 6.66 m long, 1.05 m wide and 0.21 m deep) were filled with 1500 kg soil from field B912.
Wastewater treatment tanks, each having a dimension of 1.3 m (length) × 0.7 m (width) × 0.50 m (depth) were filled with 250 L of synthetic swine wastewater which was formulated to closely resemble the nutrient profile, total ionic strength, pH, and buffering capacity of swine lagoon effluent [ 15].
Preliminary experiments were carried out on PCCPs with an inner diameter of 2 m, which were filled with pressurized water.
Micropipettes were filled with 50 m m dl-homocysteic acid (DLH) (Sigma) solution containing pontamine sky blue dye to mark the injection sites.
The electrode assemblies of a MicroRotofor focusing chamber (Bio-Rad) were filled with 0.1 M phosphoric acid (anode electrolyte) and 0.1 M sodium hydroxide (cathode electrolyte).
The micropipettes were filled with 0.1 M KCl, and the reference salt bridge with 0.1 M KCl in 2% agar.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com