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Human SGBS adipocytes (courtesy of M Wabitsch) were differentiated and cultured as previously described [18].
Out of the total 2,783 samples, M haplogroup samples (1751) were differentiated based on their specific coding region mutations.
Then, the adherent monocytes were differentiated into M Φ by culturing them with RPMI1640 10% FCS containing 100 ng/ml rhM-CSF (M2-M Φ) or 10 ng/ml rhGM-CSF (M1-M Φ).
Bone marrow cells were differentiated with M-SCF and RANKL in the presence of increasing concentrations of the drug.
The obtained data indicate three seabed facies offshore Khao Lak in water depths ranging from 5 to 35 m, which are differentiated by seafloor morphology and sediment composition.
Primary osteoclast precursors isolated from bone marrow were differentiated in the presence of M-CSF and RANKL [40].
Adipocytes were differentiated prior to incubation in PM-M microplates, such that >90% of cells contained lipid droplets.
DC were differentiated following a similar procedure where M-CSF was replaced by GM-CSF (10 ng/ml) and IL-4 (20 ng/ml).
HL-60 cells were differentiated for 36 h with 2 μ M retinoic acid.
Osteoclasts were differentiated from PBMC cultures with MIF and M-CSF, even without RANKL.
Osteoclast were differentiated from monocytes in the presence of RANKL and M-CSF (both at 25 ng/mL) [28].
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