Ten mL of ice cold sodium hydroxide (0.5 M) were added to stop the reaction after 30 min incubation.
Subsequently, different volumes (20 50 ml) of n-butylamine solution in benzene (0.01 M) were added to the test tubes.
Then, 0.6 mL of ice cold aqueous solution of NaBH4 (0.01 M) were added under vigorous stirring.
Then the following steps were performed: Aliquots of 5 mL of 2 M NaOH or different molarities of HCl (0.2 1 M) were added to the flasks.
While stirring, ANI dispersed in DI water and HCl (2 M) were added, and the reaction was continued for approximately 3 h.
A 5.0-mL Arsenazo III (0.001 M) solution and 50.0-mL monochloroacetic acid buffer (pH = 3.2; 0.1 M) were added in a 100-mL volumetric flask and diluted to the mark with distilled water after adjusting the pH to 3.2.
Another few points of apparent resistivity values from AB/2 1000 to 2000 m were added to the curve in such a fashion that apparent resistivity values increases in a linear fashion with 45° slope.
In each synthesis, l-mL EG solution of AgNO3 (0.9 M) and 0.6-mL EG solution of NaCl (0.01 M) were added into 18.4-mL EG solution of PVP (0.286 M).
Gaussian errors with standard deviation 0.01 m and 0.2° were added to the step displacements and the heading changes, respectively, and heavy-tailed Cauchy distributed errors of scale 1 m were added to the range measurements.
In a 1.5-mL Eppendorf tube, 0.78 mL of distilled water, 0.02 mL of sample appropriately diluted, and 0.05 mL of Folin-Ciocalteu reagent (final concentration 0.1 M) were added and vortexed.
After that, 15 mL of CuSO4.5H2O (0.1 M) were added to 35 mL each of 1, 0.75, 0.5, 0.25, and 0.1%% (w/v) of gelatin suspensions to get the final concentration of 0.03 M.
