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MnCl2 (200 nL, 0.3 M) was injected 1.5 mm below the dura using a calibrated microcapillary.
Subsequently, 1 mL of G-G-G aqueous solution (0.1 M) was injected into the solution.
0.5 μl KCl (1 M) was injected every 10 min within 1 h.
Subsequently, 1.5 ml of NaOH (4 M) was injected into the mixture while vigorously stirring for another 30 min.
After few minutes of stationary respiration, KCN (2 m m) was injected into the chamber.
Finally, compound M was injected into both mammary tumor susceptible MMTV-Neu mice and mouse xenograft models and was effective in reducing both tumor cell proliferation and tumor volume [ 72].
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Hyperpolarised [1-C]pyruvate (75 m), hyperpolarised [1,4-C2]fumarate (20 m) and nonhyperpolarised, unlabelled lactate (75 m) were injected into the cell suspension and single transient C spectra were acquired every second for 240 s, using a 6° flip angle pulse and a spectral width of 32 kHz.
The 100 µL of ProCA1-affi-m was injected to each xenograft by i.v. injection.
In order to measure the properties of sequential spikes, a depolarization pulse (longer than 100 ms) was injected into the GABAergic neurons to induce action potentials (Fig. 5A).
To elicit postsynaptic neuronal firing, a depolarizing current lasting 200-600 ms was injected into the recorded neurons across the entire auditory stimulus presentation period.
Repetitive square current pulses (10 nA, 20 ms) were injected into the C2 soma to evoke a train of action potentials at a constant frequency (10 Hz).
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