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The samples (A H numbered by 1 6) were autoclaved for 2 h and 10 m to remove any bacterial contamination.
Each sample was centrifuged at 12,000 rpm and 6 °C for 15 m to remove possible suspensions before loading the NMR tube.
The extra-container path loss samples are smoothed out using a sliding window of length 3 m to remove small-scale fading.
LiDAR metrics extracted for the analysis included the 30th, 70th and 95th height percentiles, mean height and canopy cover (percentage of returns from above a cut-off of 0.5 m to remove the understorey) as these are widely used for development of volume equations.
Cells were washed twice with ammonium formate 0.5 M to remove salt [ 16] and immediately frozen in liquid nitrogen.
The cells were collected by centrifugation at 800 g for 10 min and washed twice with PBS and once with isotonic sucrose solution (0.35 M) to remove the contaminating salts.
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Purified HSP90β (ca 70 μ M) in 40 m M HEPES, pH 7.5, 20 m M KCl, 1 m M TCEP was incubated with STCA (1.3 m M) or vehicle (acetonitrile, 0.1%, v/v) at 25 °C for 2 h, and dialysed against 40 m M HEPES, pH 7.5, 20 m M KCl, 0.5 m M TCEP to remove the remaining STCA.
This crude virus was purified by two rounds of cesium chloride (CsCl) gradient ultracentrifugation and dialyzed against buffer containing 10% glycerol, 10 m M Tris-HCl (pH 7.8), 135 m M NaCl, and 1 m M MgCl2*6H2O to remove CsCl.
At the indicated times, the reaction was stopped by washing the cells four times with 0.5 ml ice-cold PBS containing either 1 m M ALA or 1 m M GABA to remove nonspecific binding.
S/MARs were isolated by either 2 m NaCl or 25 m m LIS to remove histones thereby enabling unconstrained DNA to diffuse away from the nuclear scaffold/matrix forming a peripheral halo (Fig. 1A).
Cells were first concentrated via centrifugation, rinsed several times with Milli Q water and 1 m m EDTA to remove weakly sorbed metals, transferred to Teflon Savillex vials, and digested in ultrapure HNO3 and HF.
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