These peptides were chosen on basis of homology between keratin 17 and streptococcal M protein and selected for HLA-Cw6 binding.
Its potential excision might bear relevance for vaccine development, because vaccines targeting M-protein might select for M-protein-negative variants that still carry other virulence determinants.
Finally for vaccine development, because a strategy targeting the products encoded by the 47-kb SSR, e.g. M-protein, might select strains having lost the whole region, thus generating M-protein-negative strains that still carry prophage-encoded toxins and adhesin genes.
A set of query protein sequences Q={ P1, P2,…, P M }, is created by randomly selecting M proteins, where M is equal to the user-specified number of proteins to use for creating the training set.
NaCl with a concentration of 1 M was selected for future work as a high removal percentage of DNA and a low protein loss were obtained.
Then m features (m << M) are selected randomly.
The m ′ genes, m ′≈0.6* m, are selected at random and with replacement.
M, protein marker; 1 9, different batches of purified hLF.
Ghosh, P. Variation, indispensability, and masking in the M protein.
