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Maximum removal was typically at 50 m of buffer width.
All plots were separated by 2 m of buffer zones without any fertilization.
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Stationary-phase promastigote forms were fixed overnight in 2.5% glutaraldehyde, 4% of sucrose (w/v), 4% of paraformaldehyde and 5 mM of CaCl2 in 0.1 M of cacodylate buffer, and post-fixed with 1% osmium tetroxide and 0.8% potassium ferrocyanide in 0.1 M of cacodylate buffer for 1 h.
Then, the residual nanoparticles were washed with 0.1 M of phosphate buffer saline and purified by centrifugation.
We first mixed 200 μl of AGuIX nanoparticle solution (10 μmole of AGuIX) with 100 μl of 0.5 M of NH4OAc buffer (pH = 5.5).
The effect of changing the ionic strength of phosphate buffer on the selectivity and retention times of the test solutes was investigated using mobile phases containing concentration of 0.0075- 0.04 M of phosphate buffer.
The highest activity of the purified rHLZ was at pH 7 when the salt concentration was 0.05 M of the buffer.
Infected sand flies were harvested, incubated in ice-cold PBS, fixed with 2.5% glutaraldehyde, 4% of sucrose (w/v), 4% of paraformaldehyde and 5 mM of CaCl2 in 0.1 M of cacodylate buffer and embedded in paraffin.
Antigen retrieval for immunofluorescent staining was performed in 100 m m of citrate buffer (pH 6) heated in an autoclave.
The reactions were performed at 50°C in 0.05 M of citrate buffer (pH 4.8) at 150 rpm.
The cell debris was resuspended in 0.1 M of KPi buffer (pH 7.0) containing a cocktail of protease inhibitors (Roche) and centrifuged for 30 min at 10,000 g.
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