Sentence examples for m m mannitol from inspiring English sources

The serosal bathing solution contained 10 m m glucose, which was osmotically balanced on the mucosal side with 10 m m mannitol.

Briefly, 2 × 10 cells were sonicated in 20 m M HEPES buffer containing 1 m M EGTA, 210 m M mannitol, and 70 m M sucrose.

Other growth conditions included the addition of 200 m m mannitol to the standard medium and changing the pH of the medium from 6·0 to 5·0.

The branching root hair phenotype in the vti13 mutant became more severe when 200 m m mannitol was added to the growth medium (Fig.  1).

The reaction was stopped with 3 ml ice-cold buffer 1 (5 m M TrisHEPES, 50 m M mannitol, 0.25 m M MgCl2 pH=7.5).

After centrifugation, the resulting pellets were resuspended in buffer B (10 m M KH2PO4, pH 7.2, 0.3 m M mannitol, 0.1% bovine serum albumin).

The transport was stopped by transferring the samples in 1 ml stop solution (50 m m D-mannitol, 50 m m TRIS-Base, pH 7.0).

ABCC11 and control membrane vesicles, respectively (50  μg; Gibco, Darmstadt, Germany), were prepared in buffer containing 50 m m D-mannitol, 50 m m TRIS-Base, pH 7.0, 8 m m K2CO3, 10 m m MgCl2, 10 m m phosphocreatine, 4 m m ATP or AMP, 75 mU phosphocreatine kinase and radiolabelled substrate in a total volume of 75  μl.

In regards to the washing buffer of B. subtilis 168 the composition is 0.5 M sorbitol, 0.5 M mannitol, 10% glycerol.

After adding 60 μl of polyethylene glycol solution containing 40% (w/v) PEG4000 (Sigma-Fluka, MO), 0.2 M mannitol and 0.1 M CaCl2 to the wells, the plate was vortexed with a digital vortex mixer (GENIE2, Scientific industries) at 900 rpm for 15 sec.

Briefly, the hearts were collected, rinsed in ice-cold isolation buffer (0.225 M mannitol, 0.075 M sucrose, 1 mM EGTA, pH 7.4), and cut into small pieces.