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One example is the barn owl, Tyto alba, which can accurately locate prey up to 7 m in complete darkness (Iwaniuk et al. (2006).
Latrunculin A (LatA, Sigma-Aldrich) was diluted to 0.4 μ m in complete culture medium containing 0.2% FBS and applied in a time-dependent manner.
Confluent HU-V-EC cells were incubated with three doses of VEGF115-CPG2(Q)3-H6, CPG2(Q)3-VEGF109-H6, or CPG2(Q)3-H6 (50, 5, or 0.5 n M) in complete growth medium for 30 min, after which the unbound proteins were removed by rinsing × 3.
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Sample 4: cells without cytokines and 10-5 M AcSDKP in complete medium at 37°C.
Exposure of cultured cancer cells to UCN-01 (250–1000 n M) resulted in complete abrogation of phosphorylation at Ser473 and Thr308 residues of Akt, both of which are essential for full activation of Akt kinase activity (Amornphimoltham et al, 2004; Kondapaka et al, 2004).
Following 48 h exposure to vehicle or androgen ligands, the cells were stained with 100 n m MitoTracker Green FM (MTG) in complete medium prepared using phenol-red free DMEM for 30 min at 37°C (75).
A tsunami flow depth of 2 m usually resulted in complete destruction of these types of structures.
Sample 3: cells with cytokines and 10-9 M AcSDKP-NH2 in complete medium at 37°C.
Addition of 350 μ M bestatin resulted in complete inhibition of the FCS-induced invasion (P<0.05).
Sample 6: cells without cytokines and 10-9 M AcSDKP-NH2 in complete medium at 37°C.
Cells were then stained with 5 µ m JC-1 in complete medium prepared with phenol-red free DMEM for 40 min at 37°C.
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