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To prove this, he tested 50 serum samples from 50 people using leukoagglutination and a leukocyte lysis test.
The culture isolation together with the lysis test usually takes three days [7], [8].
This qPCR method utilizing phages φA1122 [7], [48], [49], [51] and L-413C [52] [54]]–[54] is a reasonable alternative to a standard phage lysis test.
The bacteriophage lysis test has been an integral part of Y. pestis detection and identification and bacteriological diagnosis of plague for about 80 years [7], [8], [47] [50].
In a 1966 study (5), sera from persons with pyrexia and jaundice were tested by the agglutination lysis test for leptospiral antibodies.
Nevertheless, in 32 critically ill patients with and without sepsis, we observed a significant correlation (r = 0.45, P < 0.001) between CRP concentrations at ICU admission and fibrinolysis assessed by the euglobulin lysis test [ 60].
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AY1 and its nano-conjugate, AY1-AgNP was non-toxic, with only 5% of cell lysis tested upto a concentration of 100 μM.
We propose qPCR with the use of both φA1122 and L-413C as a reasonable alternative to routine phage lysis tests for detection and identification of Y. pestis.
The objective of this work was to develop an alternative to conventional phage lysis tests – a rapid and highly sensitive method of indirect detection of live Y. pestis cells based on quantitative real-time PCR (qPCR) monitoring of amplification of reporter Y. pestis-specific bacteriophages.
Laboratory methods included Gram stain and culture of suspicious colonies grown on blood agar plates, and beta-lactamase, motility, and gamma-phage lysis testing.
The previously described clotting and lysis (CL) test with our original computer software was used in present study [ 11].
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